ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
a values, the pH on the cell stage has a special effect on Each individual solute’s retention time, allowing us to find the optimum pH for effecting an entire separation with the four solutes.
Transforming the cell phase’s composition because the separation progresses is one Alternative to this problem. To get a reversed-period separation we use an Preliminary cell phase that is additional polar. Given that the separation progresses, we alter the composition of cell period to make sure that it results in being considerably less polar (see Determine 12.5.six
A reversed-period HPLC separation is completed using a cell period of 60% v/v drinking water and forty% v/v methanol. What is the mobile period’s polarity index?
모든 과학 분야에서 과학자들을 지지하는 기반이 되는 기술로, 장치뿐만 아니라 컬럼이나 그 활용 방법 등도 날마다 업데이트되고 있습니다.
The detector monitors the eluent and generates a sign, that's normally in the form of a chromatogram, that is a graphical illustration of compound concentration with time.
By thoroughly taking into consideration Just about every action of your HPLC Assessment system, from sample planning to knowledge interpretation, laboratory personnel can ensure exact, reliable, and successful separation of elements in complex mixtures.
-hydroxybenzoic acid—on the nonpolar C18 column making use of an aqueous buffer of acetic acid and sodium acetate since the mobile section. The retention instances for these weak acids are shorter when utilizing a fewer acidic mobile period due to the fact Each and every solute is current in an anionic, weak foundation form that is certainly a lot less soluble inside the nonpolar stationary section.
This causes diverse elution rates for the different components and leads to the separation of the parts as here they stream out the column. As compared to column chromatography, HPLC is highly automated and extremely delicate.
Switching the mobile stage’s polarity index variations a solute’s retention aspect. As we figured out in Chapter twelve.three, nonetheless, a improve in k is just not a good way to boost resolution once the First price of k is greater than ten.
, as an example, demonstrates retention times for four weak acids in two mobile phases with approximately similar values for (P^ primary ). Even though the get of elution is the same for the two cellular phases, Every solute’s retention time is impacted otherwise by the selection of natural and organic solvent.
검토 read more 중에서 컬럼이나 이동상 등 여러 조건의 조합은 분석 가능성의 큰 영향을 미칩니다.)
A quantitative HPLC Examination is often much easier than the usual quantitative GC Investigation mainly because a set quantity sample loop gives a far more specific and accurate injection.